n Cardiovascular Journal of South Africa - Cryopreservation of platelets at the end of their conventional shelf life leads to severely impaired function : cardiovascular topic




Storage time for platelet concentrates (PCs) is limited to five days due to 'aging' of the platelets and an increasing risk of bacterial proliferation. Storage time can be prolonged by cryopreservation. We investigated <I>in vitro&lt;/I&gt; function of six consecutive PCs at the end of their conventional shelf life followed by cryopreservation for 24 hours. Spontaneous, adenosine diphosphate (ADP)-induced and collagen-induced activation before and after cryopreservation were determined by flow cytometry. Additionally, ADP- and collagen-induced aggregation was measured. After cryopreservation two-thirds of the platelets were spontaneously activated, twice as many as before the procedure (&lt;I&gt;p&lt;/I&gt; < 0.001). ADP-induced activation was significantly reduced (&lt;I&gt;p&lt;/I&gt; = 0.014). Collagen-induced activation was unchanged. Aggregation stimulated by ADP and collagen was significantly reduced (&lt;I&gt;p&lt;/I&gt; = 0.005 and p = 0.009, respectively). Our results show severely impaired <I>in vitro&lt;/I&gt; function of platelets after storage at 22°C for five days followed by cryopreservation. Cryopreservation of PCs after a storage time of five days cannot be recommended.


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