n Cardiovascular Journal of Africa - Filamin C : a novel component of the KCNE2 interactome during hypoxia : cardiovascular topics
|Article Title||Filamin C : a novel component of the KCNE2 interactome during hypoxia : cardiovascular topics|
|© Publisher:||Clinics Cardive Publishing|
|Journal||Cardiovascular Journal of Africa|
|Affiliations||1 Stellenbosch University, 2 Stellenbosch University, 3 Stellenbosch University, 4 Stellenbosch University, 5 Stellenbosch University and 6 Stellenbosch University|
|Publication Date||Jan 2016|
|Pages||4 - 11|
|Keyword(s)||Arrhythmia, Filamin C (FLNC), Hypoxia, KCNE2 and LQTS|
Aim:KCNE2 encodes for the potassium voltage-gated channel, KCNE2. Mutations in KCNE2 have been associated with long-QT syndrome (LQTS). While KCNE2 has been extensively studied, the functions of its C-terminal domain remain inadequately described. Here, we aimed to elucidate the functions of this domain by identifying its protein interactors using yeast two-hybrid analysis.
Methods: The C-terminal domain of KCNE2 was used as bait to screen a human cardiac cDNA library for putative interacting proteins. Co-localisation and co-immunoprecipitation analyses were used for verification.
Results: Filamin C (FLNC) was identified as a putative interactor with KCNE2. FLNC and KCNE2 co-localised within the cell, however, a physical interaction was only observed under hypoxic conditions.
Conclusion: The identification of FLNC as a novel KCNE2 ligand not only enhances current understanding of ion channel function and regulation, but also provides valuable information about possible pathways likely to be involved inLQTS pathogenesis.
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