oa Cardiovascular Journal of Africa - The effect of torbafylline on capillary morphology in primate skeletal muscle after ischaemic reperfusion injury
Electron microscopic and semi-quantitative morphologjcal methods were employed to determine the effect of torbafylline, a new methylxanthine derivative, on the response of capillaries to ischaemia and subsequent reperfusion Sixteen vervet monkeys under general anaesthesia were studied. A pneumatic tourniquet was applied to a hind limb for 3 hours. Eight animals received torbafylline (20 mglkg) by intravenous infusion prior to tourniquet application. Open muscle biopsies were taken from the tibialis anterior before tourniquet application, just before tourniquet release and after 6, 12 and 24 hours of reperfusion. All specimens were prepared for transmission electron microscopy. The capillaries in all pre-tourniquet specimens were normal. Endothelial cell oedema, abnormal cytoplasmic organelles and altered levels of pinocytosis occurred in various numbers of capillaries in each experimental specimen. A numeric Index was created to descriptionbe the seventy of capillary pathomorphology. Torbafylline was shown significantly to reduce the severity of capillary pathomorphology during reperfusion.
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