oa Cardiovascular Journal of Africa - Serial morphometric changes in skeletal muscle fibres during reperfusion
A histochemical, morphometric method was employed for sequential investigation of the changes occurring in primate skeletal muscle during the first 24 hours of reperfusion following 3 hours of tourniquet-induced ischaemia. While there was a general reduction in mean fibre diameters after ischaemia, fibre types 1, 2A and 2B were found to respond differently to reperfusion over the experimental period. Type 1 fibres were significantly enlarged after 6, 12, 18 and 24 hours of reperfusion, with type 2A fibres being significantly enlarged after 12, 18 and 24 hours. The trend of enlargement for type 2B fibres did not reach statistical significance. The degree of oedema, expressed as a percentage increase in fibre diameter, varied in the different fibre types, being greatest in type 1 fibres and least in type 2B. These differences in response may reflect the fibre type's metabolic dependence on oxygen. This study provides a baseline of morphometric changes in the three main fibre types following a fixed period of ischaemia and reperfusion. The model may prove useful in the evaluation of pharmacological protection of ischaemic skeletal muscle.
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