oa South African Journal of Chemistry - The determination of degradation products of lewisite and / or mustard gas in water by high performance liquid chromatography
|Article Title||The determination of degradation products of lewisite and / or mustard gas in water by high performance liquid chromatography|
|© Publisher:||South African Chemical Institute (SACI)|
|Journal||South African Journal of Chemistry|
|Author||Li Shanmao, Li Wei, Zuo Boli, Yue Lijun and Wu Qinpei|
|Publication Date||Jan 2005|
|Pages||82 - 85|
|Keyword(s)||2, 2'-dichlorodiethyl sulphoxide, 2, 2'-dihydroxyethyl sulphide, 2-chlorovinylarsonic acid, 2-chlorovinylarsonous acid and High performance liquid chromatography|
Lewisite (L) and mustard gas (HD) are highly toxic vesicant warfare agents that are very sparingly soluble in water and thereby converted quantitatively to the stable and soluble degradation products 2-chlorovinylarsonous acid (CVAA), 2-chloro vinylarsonic acid (CVAOA), 2, 2'-dihydroxyethyl sulphide and 2, 2'-dichlorodiethyl sulphoxide. A new method based on reversed-phase high performance liquid chromatography (RP-HPLC) has been developed for the simultaneous detection of CVAOA, CVAA, 2, 2'-dichlorodiethyl sulphoxide, 2, 2'-dihydroxyethyl sulphide. The effects of eluent and pH on the separation efficiency were studied. UV spectra of CVAOA, CVAA, 2, 2'-dichlorodiethyl sulphoxide and 2, 2'-dihydroxyethyl sulphide were recorded. Good separation was achieved by HPLC using a 250 x 4.6 mm column with 5 µm ODS C18 after optimization of all relevant parameters. The calibration curves of CVAOA, CVAA, 2, 2'-dichlorodiethyl sulphoxide and 2, 2'-dihydroxyethyl sulphide showed high linearity over a concentration range of 5-500, 2-500, 5-500, 50-1000 mg L-1, respectively. The detection limits at a signal-to-noise ratio of 3 were 0.001, 0.2, 2, 20mgL-1. The method may be beneficial for studying the distribution of lewisite- and/or mustard gas and their degradation products in the environment.
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