oa South African Journal of Chemistry - Supercritical fluid extraction of seed oil from Chinese licorice Fisch.) : chemical composition and antibacterial activity

Volume 60, Issue 1
  • ISSN : 0379-4350



Seed oil from Fisch. was extracted by supercritical fluid (CO) extraction. The oil was analysed by GC-MS after methylation. Compounds were identified according to their mass spectra (EI, 70 eV) by comparison with authentic reference substances and literature data. Five fatty acids were identified, with linoleic acid (24.3%) and α-linolenic acid (25.51%) being the main constituents. The effects of extraction time, pressure, temperature and CO flow rate on seed oil yield were investigated.Results showed that, under dynamic extraction time of 1.5 h, pressure 30 MPa, temperature 50 °C and CO flux of 10 kg h-1, the oil yield was 2.09% (m/m). The method was efficient for the extraction of this oil. Antibacterial activity of the oil was checked against Gram-negative and Gram-positive strains, evaluated by investigation of MIC and MBC. The seed oil of did not exhibit an antibacterial effect against the tested strains in our test system.

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