oa South African Journal of Chemical Engineering - Saccharification of bamboo by dilute acid pretreatment and enzymatic hydrolysis for cellulosic ethanol production

Volume 19, Issue 1
  • ISSN : 1026-9185



In this research, dilute sulphuric acid pretreatment for enzyme saccharification using herbaceous lignocellulose, giant bamboo, was investigated for the purpose of studying its potential as feedstock for ethanol production. Bamboos are giant woody, tree-like, perennial evergreen C4 grasses with more than 70 genera and about 1000 species. Bamboos grow naturally in tropical, subtropical, and temperate regions around the world. The central composition design was employed to optimize the pretreatment parameters, pretreated temperature range from 178 to 205 °C, residence time range from 1 to 15 minutes and acid concentration range from 0.14 to 0.44 g/ 100 g material, at reactor loading of 30%w/v (solid weight/liquid volume) in the small scale tubular reactors. The pretreated solids were enzymatically hydrolysed using enzyme loading 15 FPU/g WIS (water insoluble solid) at 50 °C for 72 h. Up to 50% glucose and 83% xylose were released from bamboo by pretreatment and enzymatic hydrolysis.

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