n African Entomology - Genetic diversity of potato aphid, Macrosiphum euphorbiae, populations in Tunisia detected by RAPD
|Article Title||Genetic diversity of potato aphid, Macrosiphum euphorbiae, populations in Tunisia detected by RAPD|
|© Publisher:||Entomological Society of South Africa (ESSA)|
|Author||F. Raboudi, H. Makni and M. Makni|
|Publication Date||Mar 2011|
|Pages||133 - 140|
|Keyword(s)||Faculte des sciences de Tunis, Genetic diversity, Institut Superieur de l'Animation pour la Jeunesse et la Culture, Macrosiphum euphorbiae, Population structure and RAPD|
In this study, the genetic structure of 15 populations of the potato aphid, Macrosiphum euphorbiae Thomas (Hemiptera: Aphididae), from Tunisia was characterized using RAPD markers. Five decameric random primers revealed 113 polymorphic loci that were used to estimate population structure with an average of 22.6 polymorphic bands per primer. Analysis of molecular variance (AMOVA) showed significant (P < 0.001) genetic diversity among populations (78.16 %) rather than within populations (21.84 %) that has been confirmed by Fst and Nm values. A cluster analysis based on genetic distance revealed that the 15 populations were divided into three groups. Populations from the south were differentiated into one group irrespective of host plant, whereas the other populations were distributed between two groups independently of the geographic origin and host plant. These results represent the first evidence for genetic structuring in M. euphorbiae.
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