n African Entomology - Eukaryotic expression and biochemical characterization of intestinal mucin SeIIM8 from (Hübner) (Lepidoptera: Noctuidae)




The invertebrate intestinal mucin is a major protein constituent of the peritrophic membrane. A SeIIM8 identified from larvae contains a 16-amino acid signal leading sequence and six chitin-binding domains with one intervening mucin-like domain and one Aps-Lys-rich domain. Prediction of -glycosylation indicated that the amino acid residues from 97 to 244 were potential -glycosylation sites. Recombinant SeIIM8 expressed in baculorvirus-infected insect cell lines binds chitin .


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