n African Entomology - Molecular phylogenetic analysis of the subgenera and (Diptera: Culicidae) based on nuclear ribosomal sequences




Phylogenetic relationships among the subgenera and were inferred from the (36 species) and (57 species) fragments of the 28S ribosomal DNA. Phylogenetic trees were reconstructed using parsimony, maximum likelihood and Bayesian methods. The resultant molecular phylogeny matched the classical morphological taxonomy reasonably well, and also resolved some ambiguities in the morphological taxonomy. Although a molecular phylogeny based on the region did not change the traditional morphological character-based arrangement of anopheline taxa, some differences from the traditional arrangement were apparent in a phylogeny based on fragment variation. This could, however, be due to the shorter length of the fragment used in this study. Both the and data support the view that the subgenus and the series are basal to the subgenus . The data suggest a sister-group composed of + ( + ). The topology of the tree based on variation supports the monophyly of all four series of the subgenus . In addition, the tree based on variation suggests that the series is monophyletic, but additional species are required to support both these hypotheses. This kind of molecular approach, in combination with the existing morphological taxonomy, could help to resolve phylogenetic relationships among the anopheline mosquitoes.


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