n African Entomology - Effect of relative humidity on entomopathogens infection and antioxidant responses of the beet armyworm, (Hübner)

Volume 22, Issue 3
  • ISSN : 1021-3589



High relative humidity is associated with high mortality in insects due to the infection with entomopathogens. However, the mechanism is still not fully understood. In this study, bioassay for nucleopolhedrovirus (SeNPV) and antioxidant enzyme activity, total antioxidant capacity, and hydroxyl radical scavenging capacity were measured in (Hübner) larvae under three relative humidities (RH) conditions (60-70%, 75-85%, and 90-100%). Results showed that humidity significantly affected the toxicity of SeNPV against larvae, and the LD (lethal dose 50%) of SeNPV against larvae was 1539.8 (60-70% RH), 1110.4 (75-85% RH) and 567.6 PIBs/g (90-100% RH). The activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX) activities of larvae were significantly lower in those subjected to high humidity than those in the control humidity group (60-70% RH). The capacity of total antioxidant and hydroxyl radical scavenging decreased with the increasing of relative humidity. These results suggest that high RH may cause a decrease in the antioxidant system of larvae, which may explain high RH is always associated with high mortality in insects due to infection with pathogens.

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