n African Entomology - Virulence and pathogenicity of recombinant nucleopolyhedroviruses expressing a scorpion toxin and an insect enzyme on (Lepidoptera: Noctuidae) larvae

Volume 22, Issue 3
  • ISSN : 1021-3589



Bioassay experiments for investigating the potential of recombinant nucleopolyhedroviruses expressing a scorpion toxin (AaIT), and a juvenile hormone esterase from (Linnaeus) (AcMsJHE) in killing first instar larvae of (Hufnagel) were tested in comparison with the wild type virus (AcMNPV). Protein expression and viral replication in insect were determined using SDS-PAGE and agarose gel electrophoresis. Results of lethal concentrations indicate that AaIT was the most efficient virus compared with AcMsJHE or AcMNPV. Median lethal concentration of AaIT was 4.0x105 PIBs/ml compared with 7.0x105 and 7.6x105 PIBs/ml for AcMsJHE and AcMNPV, respectively. AaIT killed the treated first instar larvae of markedly faster than AcMsJHE or AcMNPV, and significantly affected third instar larval weight after 96 hours post-infection (h.p.i.), which the LTs of AaIT were 94 and 89 h.p.i. when 1 x 106 and 1 x 108 PIBs/ml were used, respectively. SDS-PAGE for protein separation and agarose gel electrophoresis for DNA fragment visualization after restriction enzymes cut indicate that recombinant viruses might be replicated and expressed the inserted genes in larvae. Protein profile showed differences among larval homogenate samples infected with recombinant viruses and wild type virus compared with control. DNA fragments of recombinant viruses run on 1% agarose gel indicate that there are different size and absence fragments when the total genome cut with RI or I. Data of this work illustrated the efficiency of recombinant virus with a scorpion toxin gene on , which indicate that the use of this virus within integrated pest management programme for control may be useful.

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