n African Entomology - Evaluating triethylamine in the anaesthesia of Anopheles gambiae : short communications
|Article Title||Evaluating triethylamine in the anaesthesia of Anopheles gambiae : short communications|
|© Publisher:||Entomological Society of South Africa (ESSA)|
|Affiliations||1 University of Ghana, 2 University of Ghana, 3 University of Ghana, 4 University of Ghana and 5 Tokyo Medical and Dental University, Japan|
|Publication Date||Jan 2016|
|Pages||236 - 240|
The immobilisation of mosquitoes for injection experiments is a requirement that must be achieved for the necessary time duration to complete the process. Unfortunately, the use of cold anaesthesia in hot tropical countries is not very effective and the use of CO2 anaesthesia requires continuous exposure, which can be harmful to the experimenter. To circumvent this problem we evaluated the use of triethylamine (FlyNap®) in the anaesthesia of Anopheles gambiae mosquitoes. FlyNap has been used widely in Drosophila studies, and is known to irreversibly anaesthetise mosquitoes. Very small volumes of the original FlyNap as well as different dilutions using absolute ethanol were evaluated to determine the dosage that will effectively anesthetise Anopheles gambiae female mosquitoes for the necessary duration. The results showed that the 1/10 FlyNap/absolute ethanol) dilution worked well and could be used for the anaesthesia.
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