oa African Entomology - Taxonomic significance of isoenzymes in two stem borers (Lepidoptera: Pyralidae) of maize and sorghum in Kenya
Twelve enzyme systems were electrophoretically analysed for specific differences between the larvae of Chilo orichalcociliellus (Strand) and Chilo partellus (Swinhoe) (Lepidoptera: Pyralidae). The banding patterns of three enzyme systems, glucose phosphate isomerase, aspartate amino transferase and lactate dehydrogenase were found to be useful in differentiating larvae of the two taxa and could be considered valid taxonomic criteria for the identification of these stem borers. The electrophoretic results are in agreement with the results of morphological studies based on asetose tubercles. Enzyme analysis allowed a rapid distinction of aestivating and very young larvae of the two species which had no asetose tubercles and were morphologically indistinguishable. Electrophoresis of Chilo larvae collected in maize and sorghum fields in the coastal belt of Kenya indicated that 26.8 % were C. orichalcociliellus and 73.2 % were C. partellus.
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