oa African Entomology - A metabolic neuropeptide from the corpus cardiacum of antlions (Neuroptera: Myrmeleontidae): purification and identification
|Article Title||A metabolic neuropeptide from the corpus cardiacum of antlions (Neuroptera: Myrmeleontidae): purification and identification|
|© Publisher:||Entomological Society of South Africa (ESSA)|
|Affiliations||1 Zoology Department, University of Cape Town, Rondebosch|
|Publication Date||Jan 1997|
|Pages||213 - 230|
|Keyword(s)||Flight, Golafrus oneili, Metabolic peptides, Myrmeleontidae, Neuroptera, P. inclemens, P. speciosus, Palpares immensus and Peptide isolation|
By applying heterologous bioassays to migratory locusts and American cockroaches, the corpora cardiaca of adult antlions of the genera Palpares Rambur and Golafrus Navas were found to contain factors that increase haemolymph lipids and carbohydrates respectively. Isolation of the active compound using reversed-phase liquid chromatography revealed that it is apparently identical in all the antlion species investigated. The primary structure, identified by pulsed-liquid phase sequencing using Edman chemistry and electrospray mass spectrometry, identified the compound as an octapeptide (pGlu-Val-Asn-Phe-Ser-Thr-GlyTrp-NH2), which is a typical member of the large adipokinetic hormone/red pigmentconcentrating hormone family. Such a peptide, denoted Grb-AKH, had previously been identified in certain orthopteran insects. It is suggested that the peptide provides metabolite mobilization during flight in adult antlions.
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