n Southern African Forestry Journal - Establishment of embryogenic suspension cultures of Don. : research note

Volume 2002, Issue 194
  • ISSN : 0038-2167



The development of embryonal suspensor mass (ESM) from immature embryos of <i>Pinus radiata&lt;/i&gt; on a solidified growth medium containing 0, 5 mgl &lt;sup&gt;-1&lt;/sup&gt; benzyladenine, 3, 0 mgl &lt;sup&gt;-1&lt;/sup&gt; 2, 4-dichlorophenoxyacetic acid, 500 mgl &lt;sup&gt;-1&lt;/sup&gt; casein hydrolysate and 250 mgl &lt;sup&gt;-1&lt;/sup&gt; L-glutamine was used as inoculum to establish cell suspension cultures. Regular transplants of the ESM established on the solidified medium onto a hormone-free medium ensured sustainable growth and hence a source of ESM. Conditions for optimal growth in liquid media were investigated. The cell suspension turbidity was measured and used as a growth parameter. Optimal growth and normal embryo development were obtained in a liquid medium containing maltose as the carbohydrate source at a concentration of 3% (m/v) and supplemented with 0, 5 mgl &lt;sup&gt;-1&lt;/sup&gt; 2, 4- dichlorophenoxyacetic acid. The results obtained from this study present an opportunity for large-scale propagation of <i>Pinus radiata&lt;/i&gt; .

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