n Southern African Forestry Journal - Establishment of embryogenic suspension cultures of Pinus radiata Don. : research note
|Article Title||Establishment of embryogenic suspension cultures of Pinus radiata Don. : research note|
|© Publisher:||South African Institute of Forestry (SAIF)|
|Journal||Southern African Forestry Journal|
|Author||A. Jacoby, A.J. Van der Westhuizen and A-M. Botha|
|Publication Date||Jul 2002|
|Pages||53 - 58|
|Keyword(s)||Cell suspension cultures, Embryonal Suspensor mass and Pinus radiata|
The development of embryonal suspensor mass (ESM) from immature embryos of <i>Pinus radiata</i> on a solidified growth medium containing 0, 5 mgl <sup>-1</sup> benzyladenine, 3, 0 mgl <sup>-1</sup> 2, 4-dichlorophenoxyacetic acid, 500 mgl <sup>-1</sup> casein hydrolysate and 250 mgl <sup>-1</sup> L-glutamine was used as inoculum to establish cell suspension cultures. Regular transplants of the ESM established on the solidified medium onto a hormone-free medium ensured sustainable growth and hence a source of ESM. Conditions for optimal growth in liquid media were investigated. The cell suspension turbidity was measured and used as a growth parameter. Optimal growth and normal embryo development were obtained in a liquid medium containing maltose as the carbohydrate source at a concentration of 3% (m/v) and supplemented with 0, 5 mgl <sup>-1</sup> 2, 4- dichlorophenoxyacetic acid. The results obtained from this study present an opportunity for large-scale propagation of <i>Pinus radiata</i> .
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