n Southern African Forestry Journal - shoot multiplication and rooting from seedling explants of in Zambia : scientific paper

Volume 2006, Issue 208
  • ISSN : 0038-2167



<i>Pterocarpus angolensis</i> is a savanna tree species growing mostly in the southern part of Africa. Exploitation for its medicinal and commercial uses has led to diminishing of wild populations. Developing reliable <i>in vitro</i> protocols for propagation would enable mass production of desired genotypes for domestication and conservation of <i>P. angolensis.</i> In this study, the effect of 6-benzylaminopurine (BAP), isopentenyl-adenine (2-iP) and thidiazuron (TDZ) were tested for shoot induction on cuttings from four weeks old <i>P. angolensis</i> seedlings. BAP at 5 mg/l gave higher shoot multiplication compared with 0 - 2 mg/l BAP. Although axillary buds opened on cuttings treated with 0.1 to 2 mg/l TDZ, no elongation occurred. Shoots placed on 0.5 - 5 mg/l 2-iP produced roots but no axillary shoots. Indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) were tested for ability to induce rooting of micropropagated shoots. IAA at 1 mg/l and IBA at 1 - 4 mg/l induced higher percent rooting than other auxin concentrations. Shoots on media lacking auxins also rooted well (48%), suggesting that no growth regulator was required for rooting of 4 weeks old <i>P. angolensis</i> shoots.

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