n Southern African Forestry Journal - In vitro shoot multiplication and rooting from seedling explants of Pterocarpus angolensis in Zambia : scientific paper
|Article Title||In vitro shoot multiplication and rooting from seedling explants of Pterocarpus angolensis in Zambia : scientific paper|
|© Publisher:||South African Institute of Forestry (SAIF)|
|Journal||Southern African Forestry Journal|
|Author||Exildah Chisha-Kasumu, Adam H. Price and Steve Woodward|
|Publication Date||Nov 2006|
|Pages||31 - 37|
|Keyword(s)||Auxin, Cytokinin, Micropropagation, Miombo woodland and Pterocarpus angolensis|
<i>Pterocarpus angolensis</i> is a savanna tree species growing mostly in the southern part of Africa. Exploitation for its medicinal and commercial uses has led to diminishing of wild populations. Developing reliable <i>in vitro</i> protocols for propagation would enable mass production of desired genotypes for domestication and conservation of <i>P. angolensis.</i> In this study, the effect of 6-benzylaminopurine (BAP), isopentenyl-adenine (2-iP) and thidiazuron (TDZ) were tested for shoot induction on cuttings from four weeks old <i>P. angolensis</i> seedlings. BAP at 5 mg/l gave higher shoot multiplication compared with 0 - 2 mg/l BAP. Although axillary buds opened on cuttings treated with 0.1 to 2 mg/l TDZ, no elongation occurred. Shoots placed on 0.5 - 5 mg/l 2-iP produced roots but no axillary shoots. Indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) were tested for ability to induce rooting of micropropagated shoots. IAA at 1 mg/l and IBA at 1 - 4 mg/l induced higher percent rooting than other auxin concentrations. Shoots on media lacking auxins also rooted well (48%), suggesting that no growth regulator was required for rooting of 4 weeks old <i>P. angolensis</i> shoots.
Article metrics loading...