n International SportMed Journal - Chronic hypoxia and exercise training affect the NO content and NOS activity of rat skeletal muscle : original research article
|Article Title||Chronic hypoxia and exercise training affect the NO content and NOS activity of rat skeletal muscle : original research article|
|© Publisher:||International Federation of Sports Medicine|
|Journal||International SportMed Journal|
|Author||Wenjun Ren, Xudong Yang, Xiaogang Jiang, Zheng Li and Zhenxi Zhang|
|Publication Date||Jan 2010|
|Pages||244 - 257|
|Keyword(s)||Exercise, Nitric oxide, Nitric oxide synthase and Skeletal muscle|
Background: Nitric oxide (NO) plays a critical role in the recovery stage, in maintaining a large blood flow in skeletal muscle and in promoting recovery after exercise. Research question: This study observed the NO level, NOS activity and the expression of NOS in rat skeletal muscle under normoxic and hypoxic training conditions. Type of study: Randomised controlled study. Methods: Sixty-four male Sprague-Dawley rats were randomly divided into normoxic control group, two-week training, four-week training, six-week training groups; hypoxic control group, two-week hypoxic training, four-week hypoxic training, six-week hypoxic training groups. The classic Griess method was used to test nitric oxide in the homogenate of skeletal muscle. Moreover, methods such as RT-PCR, western blot, etc. were utilised to test the change of the all types of nitric oxide synthase expression. Results: The results showed that the NO content of rat skeletal muscle was significantly increased in two, four, and six-week training subgroups compared with the control group under normoxic conditions; NO content increased in the four-weeks and six-weeks hypoxic training group compared with the hypoxic control group (p <0.05, p <0.01). Under hypoxic conditions, with increasing exercise time, the cNOS activity and the expression cNOS protein was significant different between the training group and the control group, while there was no change in iNOS expression. Conclusions: The data in this study suggested that the hypoxic exercise may promote increases in the NOS and NO levels, counteract the blood vessel contractile effect of hypoxia, and improve the relaxed tone of blood vessels.
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