n South African Journal of Plant and Soil - Development of a low-resolution genome scan marker set for the acceleration of major gene or QTL mapping in wheat

Volume 34 Number 2
  • ISSN : 0257-1862
  • E-ISSN: 2167-034X
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The traditional approach to identify targeted quantitative trait loci (QTL) or genes in a mapping study is to develop partial to comprehensive genetic maps by using a fairly large number of molecular markers. This is a cumbersome and potentially expensive process in which the result is often good coverage of areas with no significant importance. Here we have developed a low-resolution genome scan marker set (LRGS) of 104 microsatellite (simple sequence repeat) markers to identify the gene- or QTL-carrying chromosome(s) earlier in a mapping study using a very small number of markers. In doing so, cost and time are reduced. An indication of association or linkage of a marker with the trait of interest, often still below a statistically significant threshold, can then be further investigated by saturating only the putative targeted area with additional markers to produce a high-resolution map of the region potentially housing the gene/QTL. Secondly, the LRGS markers can be used in QTL mapping efforts to provide the necessary chromosome anchoring points for high-throughput marker types with often unknown chromosome positions. This approach, combined with an automated analysis platform, accelerates a mapping effort and reduces the cost associated with the molecular work.

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