oa South African Medical Journal - An improved technique for Vi agglutination

Volume 17, Issue 11
  • ISSN : 0256-9574
  • E-ISSN: 2078-5135



Vi agglutination is to date the best serological method for detecting typhoid carriers. The usual technique may present certain difficulties, which are accentuated by South African conditions. These difficulties are largely due to the lengthiness of the usual procedure. This lengthiness causes the serums, which are often not sterile, to decompose and also allows growth of contaminants. Dark-ground studies of Vi agglutination suggested that centrifugalisation would speed up the process by providing just that contact between the bacteria which is the typical pattern of Vi agglutination. In practice it proved possible, by incubating the mixtures of serum and bacteria for a very short time and then centrifugalising them at high speed, to circumvent the difficulties mentioned. This improved technique is descriptionbed in detail.

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