oa Molecular Diagnosis and Vaccines - Detection of Chlamydia pneumoniae DNA in cholesteatoma tissue by polymerase chain reaction

Volume 2, Issue 1
  • ISSN : 1687-2010



Acquired cholesteatoma is a complication of chronic otitis media that is usually associated with an intense local inflammatory reaction. Chlamydia pneumoniae is an intracellular pathogen associated with several chronic inflammatory disorders including asthma, atherosclerosis, and Alzheimer disease. To investigate the presence of C. pneumoniae in middle ear cholesteatoma, tissue samples were collected from 30 patients undergoing middle ear surgery and processed by polymerase chain reaction for detection of C. pneumoniae, in relation to other two common middle ear pathogens, Mycoplasma pneumoniae and Haemophilus influenzae. C. pneumoniae was detected in (60%) of cholesteatoma patients . Two specimens contained H. influenzae (6.7%) and three contained Mycoplasma (10%) of which two were M. pneumoniae. A significant difference was noted (p<0.001) when the frequency of C. pneumoniae was compared with other organisms. However, on comparing the frequenct C. pneumoniae with age, gender, and smoking habits, no significant difference was observed (p>0.05). On the other hand the recurrence of cholesteatoma among C. pneumoniae positive samples was 23.3% as compared to 6.7% in C.pneumoniae negative samples (p<0.05). The high frequency of C. pneumoniae in cholesteatoma tissues may indicate an important role of this pathogen in the pathogenesis of cholesteatoma. We hypothesize that the immunological background of subjects prone to cholesteatoma formation and to easy recurrence can facilitate middle ear colonization by C. pneumoniae.

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