oa Molecular Diagnosis and Vaccines - Correlation between seminal plasma soluble HLA molecules and pre-eclampsia in Egyptian women



The involvement of immune mechanisms in the etiology of pre-eclampsia has been suggested in a number of publications. Basically, a state of tolerance to the half-foreign antigens of the fetus during normal pregnancy is widely accepted to explain the success of pregnancy and non-rejection. In pre-eclampsia, this immunological tolerance might not occur. Free HLA molecules or the so called soluble HLA (sHLA) molecules are known to have an immunomodulatory function. As sHLA antigens are present in the seminal plasma, they might cause tolerance in the mother to paternal antigens. In order to test this hypothesis, we used western blotting technique to qualitatively investigate whether sHLA class I and/or sHLA-II antigens are present in seminal plasma(sp) of husbands of pre-eclamptic women and compared the results with those from a matching group of husbands of wives with normal pregnancy. We also measured the levels of sHLA class I and sHLA class II molecules in sp from husbands of pre-eclamptic and normal pregnant controls by ELISA. sHLA class I and sHLA class II were detected in all pre-eclamptic and control groups. However, the levels of sHLA class I and sHLA class II were significantly lower in seminal plasma of husbands of preeclamptic women as compared to those of normal pregnancy control group.


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