n Obstetrics and Gynaecology Forum - Vitrification of human oocytes : review article

Volume 19, Issue 3
  • ISSN : 1027-9148



Oocyte cryopreservation has been a tool in reproductive medicine for nearly two decades. Oocytes are cryopreserved either by slow freezing or vitrification, with a subsequent thawing / warming cycle.

The slow freeze method relies on initial low cryoprotectant concentrations and a slow, two-step cooling regime (seeding and equilibration). The storage device is then plunged into the liquid nitrogen.

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