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n Obstetrics and Gynaecology Forum - Vitrification of human oocytes : review article
Oocyte cryopreservation has been a tool in reproductive medicine for nearly two decades. Oocytes are cryopreserved either by slow freezing or vitrification, with a subsequent thawing / warming cycle.
The slow freeze method relies on initial low cryoprotectant concentrations and a slow, two-step cooling regime (seeding and equilibration). The storage device is then plunged into the liquid nitrogen.
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