n Obstetrics and Gynaecology Forum - Vitrification of human oocytes : review article
|Article Title||Vitrification of human oocytes : review article|
|© Publisher:||In House Publications|
|Journal||Obstetrics and Gynaecology Forum|
|Author||B.M. Tambwe, G.M. Tinney and M.L. De Beer|
|Publication Date||Aug 2009|
|Pages||101 - 102|
Oocyte cryopreservation has been a tool in reproductive medicine for nearly two decades. Oocytes are cryopreserved either by slow freezing or vitrification, with a subsequent thawing / warming cycle.
The slow freeze method relies on initial low cryoprotectant concentrations and a slow, two-step cooling regime (seeding and equilibration). The storage device is then plunged into the liquid nitrogen.
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