n Medical Technology SA - The release of interleukin-6 after Low Level Laser Therapy (LLLT) and the effect on migration and proliferation of human skin fibroblasts - an in vitro study




Low Level Laser Therapy (LLLT) is a form of phototherapy used to promote wound healing in different clinical conditions. Many different cytokines have been shown to be present in wound fluid although their detection does not necessarily correlate with biologic activity. This study aimed to establish the effect of LLLT on the release of interleukin-6 (IL-6) from normal and wounded&lt;sup&gt;2&lt;/sup&gt; human skin fibroblasts (HSF) following irradiation and the effect of IL-6 on cell migration and cell proliferation. <br>Morphologically, wounded fibroblasts responded to single exposure of 5J/cm&lt;sup&gt;2&lt;/sup&gt; with the highest rate of haptotaxis and chemotaxis indicating a stimulatory effect while 16J/cm&lt;sup&gt;2&lt;/sup&gt; showed the lowest rate indicating an inhibitory effect. Responses were evaluated by changes in cell morphology together with changes in cell proliferation using alkaline phosphatase (ALP) enzyme activity and cytokine expression using IL-6. Cell proliferation changes indicated a decrease at higher doses of 10.0J/cm&lt;sup&gt;2&lt;/sup&gt; and 16J/cm&lt;sup&gt;2&lt;/sup&gt; while a dose of 5.0J/cm&lt;sup&gt;2&lt;/sup&gt; appeared to stimulate mitochondrial activity, which leads to normalization of cell function. A dose of 5J/cm&lt;sup&gt;2&lt;/sup&gt; showed the highest rate of cell proliferation supporting morphological evidence that 5J/cm&lt;sup&gt;2&lt;/sup&gt; has a stimulatory effect on wounded fibroblasts. Interleukin-6 studies indicated that 5J/cm&lt;sup&gt;2&lt;/sup&gt; stimulates migration, proliferation, and metabolism of wounded fibroblasts to accelerate wound closure. The results indicate a direct correlation between the dose and the stimulation of cell migration, cell proliferation and the increase in the release of ALP and IL-6.


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