n Medical Technology SA - Rapid identification and typing of enterovirus isolates

Volume 18, Issue 2
  • ISSN : 1011-5528



The use of the neutralisation assay in the serotyping of unknown enterovirus isolates has been successfully applied for years since its description by Lim and Benyesh-Melnick. However, the procedure is labour-intensive and time consuming. In addition, the continuous depletion of the Benyesh-Melnick sera makes it prohibitive for most diagnostic laboratories to continue using the assay. Owing to these factors, a majority of laboratories only type for the polioviruses and report any other enterovirus isolate as "a nonpolio enterovirus". With this approach, however, important findings such as the isolation of a new enterovirus or association of a known enterovirus with a new clinical syndrome will remain unidentified. <br>In this study, a multiple-serum-pools approach similar to that described by Lim and Benyesh-Melnick (LBM) for the neutralisation test, was applied in an immunofluorescence (IMF) test for the rapid serotyping of enteroviruses. This test was able to type any unknown enterovirus isolate belonging to the different enterovirus serotypes tested in this study, in about three hours as compared to the eight days currently taken by the LBM neutralisation test.

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