n Medical Technology SA - Adaptive response after 632.8 nm laser irradiation decreases cellular damage in diabetic wounded fibroblast cells




&lt;i&gt;Background:&lt;/i&gt; Sensitisation of cells with higher doses of ionising radiation prior to the therapeutic dose induces the so-called adaptive response. This study investigated the effect of laser pre-sensitisation of diabetic wounded fibroblast cells (WS1) with Helium- Neon (He-Ne) laser irradiation. <br><i>Materials and methods:&lt;/i&gt; A He-Ne laser was used to pre-sensitise diabetic wounded WS1 cells with 10 J/cm&lt;sup&gt;2&lt;/sup&gt; and then cells received a challenging dose (CD) of 5 J/cm&lt;sup&gt;2&lt;/sup&gt; or 12 J/cm<sup>2</sup>. After a repair time of 24 hours, cellular responses were measured. &lt;br&gt;&lt;i&gt;Results:&lt;/i&gt; A decrease in DNA damage was seen when cells were pre-sensitised with 10 J/cm&lt;sup&gt;2&lt;/sup&gt; before irradiation with a CD of 5 J/cm<sup>2</sup> or 12 J/cm&lt;sup&gt;2&lt;/sup&gt; while a decrease in the cytotoxicity of pre-sensitised cells irradiated with a CD of 12 J/cm&lt;sup&gt;2&lt;/sup&gt; was also seen. &lt;br&gt;&lt;i&gt;Conclusion:&lt;/i&gt; Pre-sensitising with 10 J/cm&lt;sup&gt;2&lt;/sup&gt; prior to irradiating with 12 J/cm&lt;sup&gt;2&lt;/sup&gt; introduced beneficial effects for cellular repair indicating an adaptive response which may reverse damage caused by higher doses of LLLT.


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