n Medical Technology SA - Adaptive response after 632.8 nm laser irradiation decreases cellular damage in diabetic wounded fibroblast cells
|Article Title||Adaptive response after 632.8 nm laser irradiation decreases cellular damage in diabetic wounded fibroblast cells|
|© Publisher:||The Society of Medical Laboratory Technologists of South Africa (SMLTSA)|
|Journal||Medical Technology SA|
|Author||Alwin B. Mbene, Innocent L. Zungu, Denise H. Hawkins, Nicolette N. Houreld and Heidi Abrahamse|
|Publication Date||Jan 2006|
|Pages||21 - 24|
|Keyword(s)||Cellular damage, He-Ne laser, LLLT and Pre-sensitisation|
<i>Background:</i> Sensitisation of cells with higher doses of ionising radiation prior to the therapeutic dose induces the so-called adaptive response. This study investigated the effect of laser pre-sensitisation of diabetic wounded fibroblast cells (WS1) with Helium- Neon (He-Ne) laser irradiation. <br><i>Materials and methods:</i> A He-Ne laser was used to pre-sensitise diabetic wounded WS1 cells with 10 J/cm<sup>2</sup> and then cells received a challenging dose (CD) of 5 J/cm<sup>2</sup> or 12 J/cm<sup>2</sup>. After a repair time of 24 hours, cellular responses were measured. <br><i>Results:</i> A decrease in DNA damage was seen when cells were pre-sensitised with 10 J/cm<sup>2</sup> before irradiation with a CD of 5 J/cm<sup>2</sup> or 12 J/cm<sup>2</sup> while a decrease in the cytotoxicity of pre-sensitised cells irradiated with a CD of 12 J/cm<sup>2</sup> was also seen. <br><i>Conclusion:</i> Pre-sensitising with 10 J/cm<sup>2</sup> prior to irradiating with 12 J/cm<sup>2</sup> introduced beneficial effects for cellular repair indicating an adaptive response which may reverse damage caused by higher doses of LLLT.
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