Medical Technology SA - Volume 21, Issue 1, 2007
Volume 21, Issue 1, 2007
Author R.T. ErasmusSource: Medical Technology SA 21 (2007)More Less
When it comes to the challenges and rewards of science and medicine, the medical technologist has the best of both worlds. On the one hand, the work of the medical technologist is so critical to the practice of modern medicine that it would be virtually impossible to provide good medical care without them. On the other hand, medical technologists are intimately involved in the research arena.
Author T.J. MooreSource: Medical Technology SA 21, pp 3 –6 (2007)More Less
Stem cells are currently a hot topic in scientific and medical research circles and are fast becoming a field of interest for South African researchers. Stem cells play important roles in normal physiological and disease processes; they also have great therapeutic potential. The study of stem cells, both internationally and in South Africa, is therefore of utmost importance. However, controversy surrounds stem cells in political, religious and ethical arenas. Although the use of certain stem cells and the means by which they are attained, do contravene certain basic ethical laws, researchers have developed methods with which to ethically obtain and create stem cell lines. These cell lines can be used to further investigate and understand the biological processes of stem cells.
Source: Medical Technology SA 21, pp 8 –12 (2007)More Less
Red palm oil (RPO) has been extensively researched for its antioxidant properties, anti-cancer activities and cardiovascular protective effects. In order to test RPO as a possible in vitro scavenger of reactive oxygen species (ROS) in human sperm a suitable solvent has to be found to introduce RPO to the sperm media because of its hydrophobic nature. In this study we tried to find the best solvent for RPO with the least detrimental effects on sperm motility. RPO was dissolved in different concentrations of solvents : ethanol (75%), dimethylsulphonate [DMSO] (87.5%) and propanol glycol [PGly] (50%) to determine the ease of solubility. These solvents were also added alone to post swim-up normozoospermic samples respectively and their effect on motility was investigated. The most suitable solvent was selected and tested in combination with RPO to determine the combined effects on motility. Motility analysis was performed by computer aided sperm analysis (CASA). It was found that of the 3 solvents tested, PGly (also known as polyethylene glycol) was able to dissolve RPO at much lower concentrations than the other two solvents. Furthermore, PGly has shown to have the least detrimental effects on sperm motility. When RPO was administered in combination with PGly it was able to attenuate the harmful effects that higher dosages of PGly have on sperm motility.
Source: Medical Technology SA 21, pp 13 –18 (2007)More Less
Since the identification of Acquired Immunodeficiency Syndrome (AIDS) in developed countries in the early 1980's, efforts to control the AIDS epidemic have focused heavily on studies of the biology, biochemistry, structural biology of human immunodeficiency virus (HIV) and on interactions between viral components and new drug candidates. HIV-1 is a very complex pathogen and encodes three structural proteins, two envelope proteins, three enzymes, and six accessory proteins. Due to the great deal of emphasis placed on drug therapy in the past decade a good review regarding the structural biology of the virus can only be advantageous. This review is an attempt to integrate structural and biochemical information into a view of the HIV-1 particle as a whole, emphasizing key interactions among viral and cellular components during the viral replication cycle.