n Medical Technology SA - The demonstration of HHV-8 in Kaposi sarcoma




Kaposi's sarcoma (KS) is a common multicentric neoplastic proliferation of endothelium of the skin and oral mucosa in patients suffering of the acquired immuno-deficiency syndrome. The role of HHV-8 (human herpes virus-8) in the etiology of Kaposi's sarcoma is beyond doubt. Due to variable results reported on the presence of HHV-8 in tissue biopsies of Kaposi's sarcoma, this study was undertaken to determine the influence of different laboratory techniques on the success rate of demonstrating the viral antigen in formalin fixed, paraffin-wax embedded tissue of diagnosed cases of skin and oral mucosal Kaposi's sarcoma. Three micron thick tissue sections of routinely fixed paraffin wax blocks of 44 cases of KS were prepared for hybridization and stained by using the following kits of Dako Cytomation: Universal Peroxidase LSAB®2 (with and without Pascal pressure chamber treatment), CSA II Biotin Free Tyramide Signal Amplification System, Hybridization and Envision. This study demonstrated a wide range of positivity between the techniques employed. LSAB stained sections without Pascal pressure chamber treatment failed to demonstrate positive staining. hybridization and Pascal pressure chamber treated LSAB®2 stained sections yielded the most positive cases. Due to cost implications the latter technique was found to be the method of choice to demonstrate HHV-8 in wax embedded tissue.


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