n Medical Technology SA - The distribution of collagen types I - IV in pleomorphic adenoma




Pleomorphic adenoma (PA) is a benign neoplasm of salivary gland origin. Despite its epithelial origin, the neoplastic cells produce several mesenchymal tissue components including connective tissue ground substance, cartilage and bone. In this study we employed histochemical and immunohistochemical techniques in order to demonstrate the status of collagen type I - IV in PA. Wax blocks of 9 PA cases were retrieved from the files of the Department of Oral Pathology at the University of Limpopo. Five micron thick sections were prepared from each block and subjected to the following staining techniques : Hematoxylin and eosin, picrosirius, reticulin, Massons trichrome and the modified Pascal immunoperoxidase technique for collagen type IV. This study demonstrated collagen type IV only in the vascular and epithelial basement membranes. The capsule and coarse septa contained collagen type I. Foci of collagen type II were observed running in a perpendicular orientation with collagen type III in myxoid areas. The study further demonstrated the potential of pleomorphic adenomas to synthesize collagen type II and III, the former which may represent the matrix for future mineralized tissue deposits, Masson-Trichrome and reticulin stains were observed not to contribute to collagen typing in PA's.


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