n Medical Technology SA - The growth response of Escherichia Coli 0111 subjected to sublethal high hydrostatic pressure : peer reviewed original article
|Article Title||The growth response of Escherichia Coli 0111 subjected to sublethal high hydrostatic pressure : peer reviewed original article|
|© Publisher:||The Society of Medical Laboratory Technologists of South Africa (SMLTSA)|
|Journal||Medical Technology SA|
|Author||Dedre Olivier and Pierre Venter|
|Publication Date||Dec 2009|
|Pages||11 - 15|
|Keyword(s)||E. coli O111, High hydrostatic pressure and Sublethal injury|
Escherichia coli O111, in peptone water, was subjected to high hydrostatic pressure (HHP) of 200 MPa for 15 minutes at 8 and 50 °C. Sub-culturing was performed and growth responses evaluated at 0, 6, 18, 24, 30, 42 and 48 hours. Percentage differences between test and control cells were calculated and results showed that E. coli O111 was only sublethally injured and able to repair itself after incubation in an enrichment broth. Sub lethally injured microorganisms could still present a potential threat given that they are able to repair themselves under suitable conditions. It is advisable to consider enrichment for at least 18 hours prior to selective cultivation. The concentration of bacteria would also dictate the magnitude of the HHP being applied. Further studies should be conducted to assess the virulence of post-HHP E. coli O111, as metabolic adaptation and cell repair might also have an impact on the lipopolysaccharide (LPS) toxin structure, the ability to form biofilm, as well as the accuracy of serological assessment.
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