n Medical Technology SA - Fluence and wavelength of low intensity laser irradiation affect activity and proliferation of human adipose derived stem cells : peer reviewed original article

Volume 24, Issue 2
  • ISSN : 1011-5528



Stem cells are undifferentiated cells that divide to replenish dying cells and regenerate damaged tissue. Low Intensity Laser Irradiation (LILI) has been shown to increase stem cell viability, proliferation, migration, and protein expression. This study aimed to compare the effects of two different wavelengths, at different fluencies, on human adult adipose derived stem cells (hADSCs).

hADSCs were irradiated at 680 or 830 nm at fluencies of 5, 10 or 15 J/cm2. Cellular responses that were measured included cell morphology, viability and proliferation. Expression of stem cell markers were also determined by immunofluorescence (IF).
Indirect IF confirmed successful stem cell isolation and character with the expression of marker ALCAM (CD166). Morphologically, LILI did not induce any changes to the hADSCs as observed between control cells (0 J/cm2) and irradiated cells. hADSCs irradiated at 680 nm showed no statistical significant differences in viability and proliferation compared to non-irradiated cells at all fluencies, while irradiation at 830 nm showed a significant decrease in both viability and proliferation at 10 and 15 J/cm2 compared to both the non-irradiated and irradiated cells at 680 nm.
Irradiation of hADSCs does not appear to induce differentiation. There is a wavelength as well as a fluence dependent effect, with cells irradiated in the visible range (680 nm) and low fluence (5 J/cm2) responding better than cells irradiated in the infra-red range (830 nm) and higher fluencies (10 and 15 J/cm2).

Loading full text...

Full text loading...


Article metrics loading...


This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error