oa Southern African Journal of Epidemiology and Infection - Diversity of bla-type genes in extended-spectrum ß-lactamase-producing isolated during 2003 - 2004 at Pretoria Academic Hospital

Volume 22, Issue 1
  • ISSN : 1015-8782
  • E-ISSN: 2220-1084



Multidrug resistance is emerging in many Gram-negative bacteria like , an important cause of nosocomial infections. Increasing production of extended-spectrum beta-lactamases (ESBLs) in this species has become a growing concern, due to associations with longer duration of hospital stay and greater healthcare costs. A few studies have investigated ESBL production in bacterial isolates collected in Africa but only one included . The aim of this study was to determine the prevalence of the ß-lactamases TEM, SHV and CTX-M detected in isolated from patients with nosocomial infections at Pretoria Academic Hospital. Fifty isolates obtained from blood cultures reported to be resistant to one or more oxyimino-cephalosporins were examined. Detection of sequences coding for bla, bla and bla were performed with genomic DNA extracted from all isolates. Polymerase chain reaction (PCR) amplification of target DNA was performed and the resulting gel-electrophoresis patterns examined. TEM- and SHV-type ß-lactamases represented 10 and 18%, respectively, in isolates analysed. Isolates that tested negative for both TEM- and SHV-type ß-lactamases comprised 6% of the study population. None of the 50 isolates examined, were positive for bla.

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