oa Southern African Journal of Epidemiology and Infection - Prevalence of toxin genes in Pretoria : original research

Volume 27, Issue 4
  • ISSN : 1015-8782
  • E-ISSN: 2220-1084



The hypervirulent polymerase chain reaction (PCR) ribotype 027 strain of produces toxins A, B and a binary toxin. Toxin detection kits are commonly used in diagnostic laboratories, but have been unsuccessful in detecting all of the relevant strains, and the toxins produced. In this study, conventional PCR was used to detect the presence of the genes of toxin A, toxin B and the binary toxin of . Eighty-four frozen (collected between 2006-2007) and 13 fresh (collected in 2010) stool specimens, obtained in Pretoria, were analysed. The genes for toxin A, toxin B and the binary toxin were detected in one of the fresh stool specimens. This may have implications for healthcare facilities, and suggests the possible emergence of the highly virulent PCR ribotype 027 strain of in Pretoria. This emphasises the importance of continuous surveillance and monitoring of outbreaks.

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