oa Southern African Journal of Epidemiology and Infection - An evaluation of the diagnostic adequacy and immunocytochemistry of manual liquid-based smears in breast aspirates : original research
|Article Title||An evaluation of the diagnostic adequacy and immunocytochemistry of manual liquid-based smears in breast aspirates : original research|
|© Publisher:||Medpharm Publications|
|Journal||Southern African Journal of Epidemiology and Infection|
|Affiliations||1 Stellenbosch University, 2 Stellenbosch University, 3 Stellenbosch University, 4 Stellenbosch University, 5 National Health Laboratory Service, 6 National Health Laboratory Service, 7 National Health Laboratory Service, 8 National Health Laboratory Service, 9 Tygerberg Hospital, 10 Tygerberg Hospital, 11 Tygerberg Hospital and 12 Tygerberg Hospital|
|Publication Date||Jan 2013|
|Pages||117 - 121|
|Keyword(s)||Cell-Solutions(R), Fine-needle aspiration biopsy, Liquid-based cytology and Syner-Med(R)|
The aim of this study was to determine if the Syner-Med®/Cell-Solutions® liquid-based cytology (LBC) technique would provide adequate diagnostic material when applied to breast fine-needle aspiration biopsy (FNAB) specimens and to determine its suitability for immunocytochemistry. A prospective study was undertaken of 38 consecutive patients who underwent FNAB of breast masses in the Fine Needle Aspiration Clinic at Tygerberg Hospital, Cape Town, over a period of six months. Conventional smear cytology slides (CSC) were formulated and the material that remained in the needle was used to prepare the LBC Syner-Med®/Cell-Solutions® slides. The CSC and LBC slides were evaluated by two pathologists. The assessed parameters were cellularity, background and representative diagnostic material. Immunocytochemical stains for pancytokeratin (MNF-116) and oestrogen receptor were performed in each case. In 33 cases (87%), LBC compared favourably with CSC. Adequacy rates of 84.2% for CSC and 76.3% for LBC were found. A diagnosis was made in 78.9% of the CSC cases and in 71% of the LBC cases. The LBC slides showed excellent results, with immunocytochemical staining for MNF-116 and oestrogen receptor. The Syner-Med®/Cell-Solutions® LBC fixative and preparation method provides an alternative technique for obtaining well fixed and prepared slides that are suitable for diagnostic cytology and immunocytochemistry.
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