oa Southern African Journal of Infectious Diseases - Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory : short article
|Article Title||Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory : short article|
|© Publisher:||Medpharm Publications|
|Journal||Southern African Journal of Infectious Diseases|
|Affiliations||1 University of Pretoria, 2 University of Pretoria, 3 University of Pretoria, 4 University of Pretoria, 5 National Health Laboratory Service and 6 National Health Laboratory Service|
|Publication Date||Jan 2016|
|Pages||29 - 31|
|Keyword(s)||Aseptic meningitis, Central nervous system, Cerebrospinal fluid, Genotype and Polymerase chain reaction|
The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains. As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-like strains may be associated with aseptic meningitis.
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