oa Central African Journal of Medicine - Investigations into the recovery of viable schistosome eggs from human tissue

Volume 13, Issue 1
  • ISSN : 0008-9176



(1) A flotation method for isolating eggs from human tissue, which also preserves the original viability at death of the body, has been investigated. (2) Two per cent. saline caused satisfactory separation between eggs and tissue in homogenised material which had previously been put through a fine sieve. (3) At room temperature 4 .per cent., 3 per cent., 2 per cent. saline solutions caused a marked decrease in the original viability of eggs voided in urine. (4) At 4� C., 37� C. and room' temperature, experiments were performed on egg viability. (5) At 40 C. the egg viability decreased only slightly after six days. (6) Eggs subjected to 2 per cent. saline at 4� C. for three hours maintained their original viability; after 24 hours the loss of viability was only 1 per cent. of the original (7) Thus it is suggested that 2 per cent. saline at 4� C. is necessary to isolate eggs satisfactorily so that a true viability count is obtained.

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