oa Central African Journal of Medicine - Biotypes and serotypes among campylobacter jejuni strains in Lagos

Volume 32, Issue 6
  • ISSN : 0008-9176



Sixty-two strains of Campylobacteria jejuni isolated from 1 200children under six years with diarrhoea in Lagos, Nigeria were biotyped and serotyped. Three biochemical tests: hippurate hydrolysis, doexyribonucleic acid (DNA) hydrolysis, and growth on charcoal yeast extract (CYE) agar were used for the biotyping of the strains. Biotype 1:22 (36%) was the most prevalent, closely followed by biotype 3:18 (29%). The serotyping was performed by the slide agglutinatior technique using commercially prepared Campylobacter polyvalent antisera supplied by the National Reference Service for Campylobacter, Ottawa, Canada. The isolates 15 (24,2%) that serologically reacted with Campylobacter pool 7 antiserum consist of the most prevalent serotype, followed by those that reacted with pool 8 antiserum, 12 (19,4%) and the remaining with pool 1,2,3,4,5,6 and 9 antisera were also represented. Only two (3,2%) strains were unserotypable. The biotyping scheme descriptionbed provides separation of strains within Campylobacter jejuni/coli which is of value for epidemiological studies. Serotyping using the polyvalent campylobacter antisera is also of value, but a combination of biotyping and serotyping is likely to provide more specific epidemiological information.

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