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oa Central African Journal of Medicine - Urinary high density lipoprotein - a possible marker for glomerular proteinuria

Volume 37, Issue 7
  • ISSN : 0008-9176

 

Abstract

High - resolution two - dimension electrophoresis technique for protein with silver staining was used to characterise urinary high density lipoprotein (HDL) - apolipoproteins. Sequential ultracentrufugation method was used to isolate urinary lipoprotein particles of the same density as serum HDL. Immunostaining of electroblotted proteins further confirmed the presence of HDL-Apos in urine. HDL - Apolipoprotein A-I, A-II and C were identified in urine of normal subjects, diabetic patients and patients with biopsy proven glomerular proteinuria. An in-house ELISA method was used to quantify urinary HDL - Apo A - I. Selectivity indices were also determined.

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/content/CAJM/37/7/AJA00089176_196
1991-07-01
2019-12-11

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