oa Central African Journal of Medicine - Fluorescence microscopy in the laboratory diagnosis and assessment of pulmonary tuberculosis

Volume 8, Issue 1
  • ISSN : 0008-9176



An investigation has been undertaken to determine the efficacy of fluorescence microscopy (using auramine stain) relative to ordinary microscopy (using Ziehl�Neelsen stain) by comparing paired smears of sputum concentrates read blindly. Preliminary to comparative microscopy, the method of homogenisation of sputum was critically examined to determine whether paired smears were likely to have comparable bacillary populations. Fluorescence microscopy was shown to be superior to the Ziehl-Neelsen method in smears with scanty bacillary populations, but there was little difference in the two methods in smears with high bacillary content. The method of fluorescence microscopy is recommended for use in busy understaffed laboratories where large amounts of tuberculous materials are being handled. It is sensitive, quick to learn, easy to use and confers a great saving of time and fatigue on technicians.

Loading full text...

Full text loading...


Article metrics loading...


This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error