oa African Zoology - Studies on the motility and cryopreservation of rainbow trout (Salmo gairdneri) spermatozoa

Volume 15, Issue 4
  • ISSN : 1562-7020
  • E-ISSN: 2224-073X



The very short duration of vigorous movement (1 1/2 to 7 min) in fresh water and physiological solutions make trout spermatozoa difficult subjects for cryopreservation studies. Solutions consisting of 250 to 280 mmol sucrose and 5 to 12% dimethyl sulphoxide (DMSO) (4 parts) did not activate trout spermatozoa (1 part), but after dilution with fresh water vigorous motility could be fully restored. These sucrose-DMSO solutions were employed in cryopreservation studies. Using straws and a fast freezing - fast thawing procedure, post-thaw dilution with fresh water resulted in 25% - 60% of spermatozoa becoming motite, all with vigorous forward progression. Some existing methods for the cryopreservation of other freshwater fish spermatozoa were repeated on trout without success.

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