oa Agrochemophysica - Evaluation of acid hydrolysis procedures for the rapid determination of total non-structural carbohydrates in pasture species

Volume 11, Issue 1
  • ISSN : 0302-7112



An evaluation of existing acid hydrolysis procedures for total non-structural carbohydrates indicated that total extraction can be achieved on material milled to pass a 1 mm sieve and analysis for reducing sugars can be done directly on the hydrolysed extract without prior neutralization. Provided conditions for acid hydrolysis are carefully selected and standard samples are included in each run, results can be obtained with this rapid method of analysis, which agree well with those obtained with the much more tedious enzymic procedures.

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