oa Agrochemophysica - The effect of zinc on DNA and protein synthesis in dividing in dividing rat lymphocytes

Volume 7, Issue 1
  • ISSN : 0302-7112



Incorporation of 3H-thymidine into DNA, and I4C-leucine into protein was decreased by 90-95 % in PHA-transformed rat lymphocytes cultured in media to which had been added 1 mM of EDTA. The inhibition was effectively reversed by the addition of zinc which restored respectively 82 % and 64% of the original activities of DNA and protein synthesis. Other divalent ions also reversed the inhibition, especially with regard to protein synthesis, but their effectiveness was considerably less than that of zinc. Concentrations of zinc in excess of 0,5 mM were found to inhibit both DNA and protein synthesis. Incorporation of 3H-thymidine into DNA, and 14C-leucine into protein was decreased respectively by 34% and 26,5% in lymphocytes cultured in a zinc-deficient medium.

Loading full text...

Full text loading...


Article metrics loading...


This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error