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oa Agrochemophysica - A lectin-like toxin from the Natal round yellow bean (Phaseolus vulgaris)

Volume 8, Issue 3
  • ISSN : 0302-7112

 

Abstract

A fraction which was toxic to mice [LD60 = 2,7 (2,0-3,6) mg] upon intraperitoneal injection but having low haemagglutinating activity was isolated from Natal round yellow beans (Phaseolus vulgaris) and was characterised by a number of physico-chemical methods. The toxic fraction was found to contain both protein and carbohydrate. The protein moiety was virtually homogeneous and a large proportion of the carbohydrate could be separated from the protein by chromatography at pH 4,0 or pH 9,5. Amino acid analysis indicated the virtual absence of cystine and the presence of a relatively high proportion of aspartic acid and serine residues. A structural analogy is suggested between this toxin and the haemagglutinins previously isolated from Phaseolus vulgaris seeds.

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/content/agro/8/3/AJA03027112_313
1976-01-01
2020-11-29

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