n African Journal of Laboratory Medicine - Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda : brief report

Volume 5, Issue 1
  • ISSN : 2225-2002
  • E-ISSN: 2225-2010



This study evaluated the performance of the Kalon Biological HSV2 IgG enzyme-linked immunosorbent assay (Kalon Biological Ltd, Surrey, United Kingdom) on dried blood spots (DBS) of various dilutions compared with plasma from young women aged 18-24 years in Uganda. We estimated the sensitivity and specificity of three DBS dilutions using plasma as the reference. All three evaluated DBS dilutions yielded low sensitivities and specificities, with DBS 1:2 yielding the highest concurrence. Other herpes simplex virus type 2 assays should be examined with regard to their utility for testing DBS.

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