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n African Journal of Laboratory Medicine - Verification of chemistry reference ranges using a simple method in sub-Saharan Africa : original research

Volume 5, Issue 1
  • ISSN : 2225-2002
  • E-ISSN: 2225-2010
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Abstract

Chemistry safety assessments are interpreted by using chemistry reference ranges (CRRs). Verification of CRRs is time consuming and often requires a statistical background.


We report on an easy and cost-saving method to verify CRRs.
Using a former method introduced by Sigma Diagnostics, three study sites in sub- Saharan Africa, Bondo, Kenya, and Pretoria and Bloemfontein, South Africa, verified the CRRs for hepatic and renal biochemistry assays performed during a clinical trial of HIV antiretroviralpre-exposure prophylaxis. The aspartate minotransferase/alanine aminotransferase, creatinineand phosphorus results from 10 clinically-healthy participants at the screening visit were used. In the event the CRRs did not pass the verification, new CRRs had to be calculated based on 40 clinically-healthy participants.
Within a few weeks, the study sites accomplished verification of the CRRs withoutadditional costs. The aspartate aminotransferase reference ranges for the Bondo, Kenya site and the alanine aminotransferase reference ranges for the Pretoria, South Africa site required adjustment. The phosphorus CRR passed verification and the creatinine CRR requiredadjustment at every site. The newly-established CRR intervals were narrower than the CRRs used previously at these study sites due to decreases in the upper limits of the reference ranges. As a result, more toxicities were detected.
To ensure the safety of clinical trial participants, verification of CRRs should bestandard practice in clinical trials conducted in settings where the CRR has not been validated for the local population. This verification method is simple, inexpensive, and can be performed by any medical laboratory.

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/content/ajlab/5/1/EJC197652
2016-01-01
2019-10-21

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