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n African Journal for Physical Health Education, Recreation and Dance - Mycobacterium tuberculosis genotypes and drug susceptibility patterns in Limpopo Province, South Africa

Volume 21 Number 4.1
  • ISSN : 1117-4315

Abstract

Tuberculosis is a worldwide health burden with high morbidity and mortality rates. About 8 million people are infected annually, with 1.5 million deaths reported in 2013. The disease is complicated by its high drug resistance and transmission rate. Information about drug resistance, evolutionary genetics and transmission of Mycobacterium tuberculosis strains is limited in Limpopo Province. The aims of the study were therefore to characterise and genotype Mycobacterium tuberculosis in sputum samples from patients living in Limpopo Province and to establish the main families of Mycobacterium tuberculosis circulating in the province and their main genetic mutations. Two hundred and seven (207) acid-fast positive samples were collected from National Health Laboratory Services in Polokwane. Geno Type MTBDRplus and Real-Time PCR were used to determine resistance genes and families, respectively. Beijing and non-Beijing genotypes were detected in 27.6% and 72.4% of the strains, respectively. Co-infection with both genotypes was detected in one sample. Of the 207 Mycobacterium tuberculosis strains tested, 28 (13.5%) exhibited drug resistance, of which 14 isolates (50%) were multi-drug resistant. Monoresistant strains to rifampicin constituted 25% (7 isolates) and the other 25% (7 isolates) were mono-resistant to isoniazid. Rifampicin mutations were identified in codons 516 (43%), 526 (33%) and 531 (38%) of the rpoB gene. Isoniazid mutations were identified in codons 15 (19%) and 16 (14%) of the inhA gene and codon 315 (62%) of the katG gene. The non-Beijing is moreprevalent than the Beijing genotype in the Limpopo Province. Drug resistance against rifampicin and isoniazid was in different genes and codons. The high rate of drug resistance calls for continuous monitoring of antimicrobial drugs and treatment default by TB victims.

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/content/ajpherd/21/Issue-41/EJC182196
2015-12-01
2019-09-18

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