1887

n Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie - Die groot verskil in chemiese samestelling en antibakteriese aktiwiteit van twee na-verwante spesies (Lamiaceaea) mag taksonomiese waarde hê : navorsings- en oorsigartikels

Volume 29, Issue 1
  • ISSN : 0254-3486
  • E-ISSN: 2222-4173

Abstract

Daar is verskeie menings oor die taksonomie van spesies en onlangse outeurs het groot veranderings aanbeveel. Sommige outeurs beskou en as variante van dieselfde spesie en ander as twee aparte spesies. spesies word wyd gebruik vir medisinale doeleindes. Heelwat van die gebruike is vir toestande wat met bakteriese infeksies verband hou. Die chemiese samestelling en antibakteriese aktiwiteit van verskillende plante van twee populasies van die spesies is ondersoek om te bepaal of hierdie kenmerke van taksonomiese waarde kan wees.


Beide die samestelling van pigmente geskei deur dunlaagchromatografie en van verbindings wat sigbaar geword het na behandeling van chromatogramme met vanillien-swawelsuur het aangedui dat daar groot verskille tussen plante van die twee populasies bestaan.
Die antibakteriese aktiwiteit van asetoonekstrakte is bepaal deur die minimum inhiberende konsentrasie (MIK) te bepaal deur middel van 'n tweevoudige verdunningsreeks mikroplaat metode met tetrasoliumviolet as aanduider van bakteriese groei. As toetsorganismes is (American Type Culture Collection 29213), (ATCC 27853), (ATCC 25922) en (ATCC 21212) gebruik. Daar was groot verskille in die antibakteriese aktiwiteit tussen die twee spesies veral nadat die totale aktiwiteit in ml/g droë materiaal tussen die twee spesies vergelyk is. Die asetoonekstrak het verbasende goeie aktiwiteit gehad met 'n MIK (33 µg/ml) van net so goed of beter as die MIK van ampisillien, gentamisien, nitrofurantoïen, trimetroptien, sulfisoksasool teenoor . Dieselfde ekstrak het 'n MIK (15 µg/ml) van net so goed of beter as ampisillien, gentamisien, nitrofurantoïen en sulfisoksasool gehad. Daar bestaan 'n sterk moontlikheid dat 'n asetoon ekstrak van kommersiële toepassing kan vind indien daar nie stabiliteit of toksisiteit probleme is nie.
Die resultate ondersteun die beskouing dat die twee taksons as afsonderlike spesies beskou moet word.


Several species are used widely for medicinal purposes in Africa. There have been drastic changes in the taxonomic treatment of species during the past decade. Two species, and occurring in Pretoria have been considered as varieties of the same species and as different species by different authors. Because species are used widely as medicinal plants against bacterial infections, we decided to compare the chemical composition and antibacterial activity of four plants from each of two populations of the species.
The chemical composition of acetone extracts of finely ground leaves was determined by thin layer chromatography followed by spraying with vanillin-sulphuric acid. There were hardly any differences between plants from the same population. There were major differences between the two species in the composition of pigments separated by thin layer chromatography and for compounds visualized with the vanillin-sulphuric acid spray reagent. This supported the viewpoint that the two species should not be considered as varieties. The major differences found in chemical composition indicate that chemical parameters may play an important role in resolving taxonomic differences. Because such a small quantity of material is needed, it may be feasible to analyze one or two leaves obtained from herbarium sheets as an additional taxonomic parameter.
The antibacterial activity of the acetone extracts was determined using a two-fold serial dilution microplate method with tetrazolium violet as indicator of growth. The specific strains of the four most important nosocomial bacterial pathogens suggested by the United States National Committee for Clinical Laboratory Standards were used : (American Type Culture Collection 29213), (ATCC 27853), (ATCC 25922) and (ATCC 21212). The minimum inhibitory activity of the crude extract of was 58, 33, 113 and 15 µg/ml against the four pathogens respectively. The MIC of the extracts were 110, 95, 113 and 63 µg/ml respectively. If the total activity was calculated by dividing the quantity in mg extracted from one gram of each species with the MIC, the following values were obtained against the respective bacteria using the extract : 700, 1400, 381 and 2100 ml/g. The values for the extract were 381, 420, 420 and 700 ml/g. This means that if one gram of dried leaves of were extracted with acetone it could be diluted to 2100 ml and it would still kill .
These results not only prove the possible use of chemical and biological activity as taxonomic markers, but also the potential value of acetone extracts in treating infections with and . The activity of the crude extract against was as good as or better than the activity of ampicillin, gentamicin, nitrofurantoin, trimethroptin or sulfisoxazole. The activity against was as good as or better than ampicillin, gentamicin, nitrofurantoin or sulfisoxazole. If the acetone leaf extract of is stable and not toxic there is a good possibility of developing a commercially useful antibacterial product from it.
The results indicate the taxonomic value of chemical parameters and biological activity and support the view that the two taxa should be considered as different species.

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/content/aknat/29/1/EJC20468
2010-03-01
2019-08-18

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