n Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie - 'n PKR-opsporingsmetode vir mutasies in proteïenreseptorgene vanaf -ingewande, moontlik betrokke by Bt-weerstandbiedendheid : referaatopsomming

Volume 33, Issue 1
  • ISSN : 0254-3486
  • E-ISSN: 2222-4173



(Fuller) (Lepidoptera: Noctuidae) is a major insect pest to Bt-maize in South Africa. No study has yet been done to determine the molecular mechanism of resistance to Cry1Ab. Aminopeptidase N (1-6), cadherin and alkaline phosphatase receptor-protein genes were amplified and sequences of PCR products were determined. BLAST searches showed similarity to the respective receptor-protein genes. Mutations can thus be identified by comparing sequences of receptor-protein genes from resistant and susceptible larvae.

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