1887

n African Entomology - Isolation and functional characterisation of antibacterial peptide from the mosquito, () (Diptera: Culicidae)

Volume 24, Issue 1
  • ISSN : 1021-3589
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Abstract

One antimicrobial peptide was isolated and purified to 5.50-fold from the crude lysate of immunised larvae by (NH)SO fractionation, ion-exchange chromatography, and reverse-phase high performance liquid chromatography (HPLC). The peptide, named ACAm, was found to be heat stable below 80 °C and exhibited strong antimicrobial activities against Gram-positive and Gram-negative bacteria as well as against peptidoglycans and lipopolysaccharides of bacterial cell walls. The estimated molecular weight of ACAm by sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE), under non-reducing conditions, was 4 kDa. Using isoelectric focusing (IEF), ACAm exhibited pI in basic pH (8.4) belonging to cationic peptides.

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/content/ento/24/1/EJC185844
2016-01-01
2016-12-10

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