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oa Southern African Journal of Infectious Diseases - The use of aged stool specimens for the detection of rotavirus - original research

Volume 35 Number 1
  • ISSN : 2312-0053

 

Abstract

Background: Rotavirus is considered worldwide as one of the most important viral gastrointestinal infections, resulting in potentially life-threatening diarrhoea and death in children under the age of 5 years. Rotavirus can survive and remain infectious for long periods outside of the human body and can be easily transmitted via environmental surfaces.

Method: Stool specimens that had been collected and stored since 2010/2011 at 2°C – 8°C instead of −20°C or −80°C were analysed to determine the viability of rotavirus in these specimens after 6 years of improper storage. The specimens were analysed using simple enzyme immunoassay (EIA) methods from two different suppliers at different times throughout the period (2012–2017).

Results: The analysis showed similar detection results for the two EIA kits.

Conclusion: The rotavirus can be detected after several years of incorrect storage with EIA kits.

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/content/journal/10520/EJC-1dfb06de56
2020-06-29
2020-07-13

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